ABSTRACT
Objective To investigate the protective effect of curcumin (Cur) on mice cornea against ultraviolet A (UVA)-induced damage.Methods Together 30 C57BL/6 mice were randomly divided into 3 groups (10 mice in each group):UVA irradiation group (UVA group),UVA irradiation with curcumin treatment group (UVA + Cur group) and control group.Mice in the UVA group was exposed to UVA for 24 h,with the wavelength of 320-400 nm and the irradiation intensity of 0.05 W · cm-2.In UVA + Cur group,mice were given 30 mg · kg-1 curcumin intraperitoneally 3 days before irradiation and lasted until 48 h after irradiation.And the control group was left untreated.Then corneal opacity was evaluated and scored through slit-lamp biomicroscopy.After irradiation for 48 h,corneal tissue was collected for the detection of the expression of NF-κB by electrophoretic mobility shift assay.Results After irradiation for 48 h,the score of corneal opacity in the UVA group (3.10 ± 0.74) was higher than that of the control group (0) and UVA + Cur group (0.35 ± 0.24) (P ＜ 0.05).The expression of NF-κB in cornea was detectable at a low level in the control group (1.25 ± 0.13),but was remarkably increased in UVA group (3.98 ± 0.58) and suppressed by curcumin in UVA +Cur group (1.58 ± 0.34) (P ＜ 0.05).There was no significant difference in corneal opacity score and NF-κB expression between control and UVA + Cur group (P ＞ 0.05),but there was significant difference between control and UVA group and UVA + Cur and UVA group (all P ＜ 0.05).Conclusion Curcumin can protect cornea against UVA-induced damage via inhibiting NF-κB activation.
ABSTRACT
Objective To evaluate the optical quality in patients with meibomian gland dysfunction (MGD) by using double-pass optical quality analysis system (OQAS-Ⅱ) for offering the reference data for clinical practices.Methods A total of 50 patients with a diagnosis of MGD,who were divided into MGD with dry eye disease (DED) group (n =22) and MGD without DED group (n =28) according to the results of tear film break-up time (BUT) and Schirmer I test (SIT),and another 25 healthy subjects were enrolled in this study.Then several parameters,including modulation transfer function cutoff (MTF cutoff),Strehl ratio (SR) and objective scattering index (OSI),were measured to evaluate the optical quality by using OQAS-Ⅱ.Additional clinical examination,including meibomian gland and tear film assessment were per formed and compared between the three groups.In addition,a correlation analysis was conducted among OSI,MTF cutoff,SR,BUT,SIT and meibomian gland assessment.Results Statistical differences were approached in MTF cutoff,SR,OSI of the three groups (all P ＜ 0.05).The MTF cutoff and SR in MGD without DED group were significantly lower than those in the control group [(31.36 + 1.83) c · deg-1 vs.(35.87 ± 1.59)c·deg-1,(0.21 +0.02) vs.(0.23 ±0.03)],but the OSI got higher [(0.57+ 0.06) vs.(0.45 ±± 0.06)] (all P ＜ 0.05).The SR and MTF cutoff in MGD patients with DED was significantly lower than those in MGD patients without DED [(27.87 ±± 3.08),c·deg-1 vs.(31.36±±1.83)c· deg-1,(0.16 ±±0.02) vs.(0.21 ± 0.02)],but OSIgot significantly higher [(0.72 ± 0.10) vs.(O.57 ± 0.06)] (all P ＜ 0.05).Among the three groups,OSI,MTF cutoff and SR showed no significant correlation with BUT,SIT and meibomian gland assessment (all P ＞ 0.05).Conclusion There is changes in visual quality parameters in MGD patients by OQAS-Ⅱ,and MGD with DED patients has significant changes than MGD patients without DED.
ABSTRACT
Objective To evaluate the optical quality in patients with meibomian gland dysfunction (MGD) by using double-pass optical quality analysis system (OQAS-Ⅱ) for offering the reference data for clinical practices.Methods A total of 50 patients with a diagnosis of MGD,who were divided into MGD with dry eye disease (DED) group (n =22) and MGD without DED group (n =28) according to the results of tear film break-up time (BUT) and Schirmer I test (SIT),and another 25 healthy subjects were enrolled in this study.Then several parameters,including modulation transfer function cutoff (MTF cutoff),Strehl ratio (SR) and objective scattering index (OSI),were measured to evaluate the optical quality by using OQAS-Ⅱ.Additional clinical examination,including meibomian gland and tear film assessment were per formed and compared between the three groups.In addition,a correlation analysis was conducted among OSI,MTF cutoff,SR,BUT,SIT and meibomian gland assessment.Results Statistical differences were approached in MTF cutoff,SR,OSI of the three groups (all P ＜ 0.05).The MTF cutoff and SR in MGD without DED group were significantly lower than those in the control group [(31.36 + 1.83) c · deg-1 vs.(35.87 ± 1.59)c·deg-1,(0.21 +0.02) vs.(0.23 ±0.03)],but the OSI got higher [(0.57+ 0.06) vs.(0.45 ±± 0.06)] (all P ＜ 0.05).The SR and MTF cutoff in MGD patients with DED was significantly lower than those in MGD patients without DED [(27.87 ±± 3.08),c·deg-1 vs.(31.36±±1.83)c· deg-1,(0.16 ±±0.02) vs.(0.21 ± 0.02)],but OSIgot significantly higher [(0.72 ± 0.10) vs.(O.57 ± 0.06)] (all P ＜ 0.05).Among the three groups,OSI,MTF cutoff and SR showed no significant correlation with BUT,SIT and meibomian gland assessment (all P ＞ 0.05).Conclusion There is changes in visual quality parameters in MGD patients by OQAS-Ⅱ,and MGD with DED patients has significant changes than MGD patients without DED.